EVALUATE THE EFFECT OF YELLOW LUPINE AND FENUGREEK EXTRACTS ON BIOCHEMICAL PARAMETERS ON COTTON LEAF WORM

Abstract

The Egyptian cotton leaf worm is considered the major pest that causes great damage to cotton plants as well as other vegetable crops in Egypt. Great efforts have been made to control this pest chemically. Insecticides of synthetic origin have been used to manage insect pests for more than 50 years. Due to the continuous use of chemical pesticides against this pest, resistance to the action of pesticides had dramatically evolved. This study was planned to identify the phenolic compounds in ethanol extracts of fenugreek and yellow lupine, on the other hand, investigate the effect of water and alcoholic extracts of fenugreek and yellow lupine on the biochemical responses of the 4th instar larvae of cotton leaf worm. In our study a recommended pesticide namely chlorpyrifos (dursban) was used as a reference. Our results indicated that seven compounds were detected in two ethanolic extracts; gallic acid (164.409 mg /ml) was the major component in fenugreek extract, while catechin was (113.705 mg /ml) the major phenolic compound in yellow lupine extract. All tested extracts showed a significant deterioration in biochemical parameters (GOT, GPT, ALP, ACP activities and total protein levels). The 10 % concentration was the best in both plants extracts, while the ethanolic extract of the fenugreek was the most effective of all the extracts used in the experiment. So, we recommended by using fenugreek and yellow lupine extracts as insecticides to control the cotton leaf worm.