Efficiency of inoculation methods for genotypes selection in corn ear rot disease studies

Sabrina Helena Araujo, O. Júnior, Luis Oswaldo Viteri, Raimundo Wagner Aguiar, E. E. Oliveira, G. R. Santos
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引用次数: 2

Abstract

The identification of resistance of plants to pathogens is crucial for the development of hybrids by breeding programs. To achieve that, it is of great relevance to establish effective inoculation methods for characterizing genotypes with adequate plant resistance levels. Several inoculation methods have been investigated in the search for resistance to corn ear rot disease. However, studies evaluating different corn genotypes cultivated under Neotropical field conditions remain unexplored. Here, we compared three inoculation methods (i.e., aspersion, injectable, and natural) of Fusarium verticillioides in corn ears, and evaluated disease severity and grain yield of 10 corn genotypes. The experiments were conducted in two consecutive experimental corn crop (i.e., 2015 and 2016) seasons located in cities with different environmental temperatures and belonging to different Brazilian states (i.e., Gurupi – Tocantins State, Itumbiara – Goiás State, Planaltina – Brazilian Federal District, and Toledo – Paraná State). We evaluated the mass of 1000 grains and the severity of the disease. Regarding the disease severity, our results showed that the artificial inoculation was more efficient in the regions of Planaltina and Toledo, not affecting grains’ mass in these localities. The severity of disease on the conditions of Toledo was similar for the ten genotypes. However, it was possible to identify two contrasting genotypes since P4285H (low severity) and 32R48YH (medium severity) exhibited significantly more disease symptoms in all other regions. Although the differences regarding the efficiency of inoculation methods are more evident in regions with milder temperatures, the results showed that the characterization of genotypes susceptible to the pathogen is more efficient in regions with higher temperatures.
玉米穗腐病研究中接种方法基因型选择的有效性
鉴定植物对病原菌的抗性对杂交育种至关重要。为了实现这一目标,建立有效的接种方法来鉴定具有适当植物抗性水平的基因型具有重要意义。为寻找玉米穗腐病的抗性,研究了几种接种方法。然而,在新热带大田条件下评价不同玉米基因型的研究仍未开展。本研究比较了三种接种方法(喷洒、注射和自然接种)在玉米穗上对黄萎病的影响,并对10种玉米基因型的病害严重程度和产量进行了评价。实验在巴西不同州(Gurupi - Tocantins州、Itumbiara - Goiás州、Planaltina -巴西联邦区和Toledo - paran州)环境温度不同的城市连续两个玉米作物试验季节(即2015年和2016年)进行。我们评估了1000粒谷物的质量和疾病的严重程度。在严重程度上,我们的结果表明,人工接种在Planaltina和Toledo地区更有效,不影响这些地区的籽粒质量。10个基因型在托莱多条件下的疾病严重程度相似。然而,由于P4285H(低严重程度)和32R48YH(中等严重程度)在所有其他地区表现出更多的疾病症状,因此有可能鉴定出两种不同的基因型。虽然接种方法的效率差异在温度较温和的地区更为明显,但结果表明,在温度较高的地区,对病原体敏感的基因型的鉴定效率更高。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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