Feasibility Of Using Orange Juice For Digestion Of Swamp Eel Viscera For Harvesting Infective Stage Larva Of Gnathostoma Spp.

Abstract

Gnathostoma spinigerum third stage larvae antigen is necessary for Western blot analysis in the diagnosis of Gnathostomiasis. Acid pepsin solution is required for digestion of eel's liver( Fluta alba ) to yield the larvae for antigen preparation. Here, he reported the feasibility of using orange juice as an alternative to enzyme pepsin in harvesting Gnathostoma spinigerum third stage larvae. From the experimental digestion, it was found that we could recover larvae from orange juice. Thus, orange juice might be another choice for recovering Gnathostoma spinigerum third stage larvae.