Effects of 220 MHz Radiofrequency Field on Sperm Quality of SD Rat

Ling Guo, Yi-Zhe Xue, Jiajin Lin, Guang-zhou An, Junping Zhang, Ke-Ying Zhang, Wei He, Huan Wang, Wei Li, G. Ding
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引用次数: 1

Abstract

Under some occupational conditions, workers are inevitably exposed to high-intensity radiofrequency (RF) field, so the effects of long-term exposure to RF field on health have already become a public concern. In this study, we investigated the effects of one-month exposure to a 220 MHz RF field at two different average power densities (50 W/m2 and 100 W/m2) on the sperm quality in male adult Sprague-Dawley (SD) rats. Sperm quality was evaluated by measuring the number, the abnormality and the survival rate of sperm. The morphology of testis was examined by hematoxylin-eosin (HE) staining. Levels of secreting factors by Sertoli cells (including glial cell line-derived neurotrophic factor (GDNF), stem cell factor (SCF), transferrin (TRF) and androgen binding protein (ABP)) and factor by Leydig cells (testosterone, T) were determined by enzyme linked immunosorbent assay (ELISA). The expression levels of the apoptosis-related protein (Caspase 3, BAX and BCL 2) in testis were assessed by Western Blotting analysis. Compared with the sham group, the sperm quality in RF groups decreased significantly in a dose-dependent manner. Levels of GDNF, SCF, TRF and ABP in testis as well as the morphology of testis, showed on obvious changes after RF exposure. The level of serum T decreased significantly in the rats treated with 50 W/m2 RF exposure and increased significantly in the rats treated with 100 W/m2 RF exposure, compared with sham group. In addition, the protein levels of Caspase 3 and the BAX/BCL 2 ratio in testis increased obviously after RF exposure, compared with sham group. These data collectively suggested that under the present experimental conditions, 220 MHz RF exposure could affect sperm quality in rats, and the disruption of secreting function of Leydig cells and increased apoptosis of testis cells induced by RF field might both account for this damaging effect.
220 MHz射频场对SD大鼠精子质量的影响
在某些职业条件下,工人不可避免地接触到高强度射频场,因此长期接触射频场对健康的影响已经成为公众关注的问题。在这项研究中,我们研究了在两个不同的平均功率密度(50 W/m2和100 W/m2)下暴露一个月的220 MHz射频场对成年雄性SD大鼠精子质量的影响。通过测量精子数量、异常情况和精子存活率来评价精子质量。苏木精-伊红(HE)染色观察睾丸形态。采用酶联免疫吸附试验(ELISA)检测支持细胞分泌因子(包括胶质细胞系来源的神经营养因子(GDNF)、干细胞因子(SCF)、转铁蛋白(TRF)和雄激素结合蛋白(ABP))和间质细胞分泌因子(睾酮,T)的水平。Western Blotting检测凋亡相关蛋白Caspase 3、BAX、BCL 2在睾丸组织中的表达水平。与假药组比较,RF组精子质量呈剂量依赖性明显下降。RF暴露后,睾丸组织中GDNF、SCF、TRF、ABP水平及睾丸形态均发生明显变化。与假手术组相比,50 W/m2射频暴露组血清T水平显著降低,100 W/m2射频暴露组血清T水平显著升高。RF暴露后,与假手术组相比,睾丸Caspase 3蛋白水平和BAX/BCL 2比值明显升高。综上所述,在本实验条件下,220 MHz射频暴露可影响大鼠精子质量,射频场诱导的睾丸间质细胞分泌功能的破坏和睾丸细胞凋亡的增加可能是造成这种损害的原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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