Hystometric evaluation of nickel chronic exposure effects on large instestine of adult Wistar male rats

Maria Luiza Assis Rodrigues, Sirlene Souza Rodrigues Sartori, Priscila Izabel Santos Totaro, Sérgio Luis Pinto da Matta
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引用次数: 1

Abstract

The ingestion of considerable amounts of water or food contaminated with nickel can be very toxic. The present work was conducted aiming to evaluate the effects of nickel exposures on ascending colon of adult Wistar male rats at hystometric level. We used 12 animals that were divided in a control (ingested uncontaminated water) and a nickel-contaminated (i.e., 25 mg de nickel/L of water) groups. Nickel chloride was offered in declorinated water and the experiment had a 56 days exposure period. A portion of the ascending colon was removed of the animals and subjected to hystological labelling processes using blue toluidin (for general hystometric description), Alcian Blue (AB, for acid mucins) and periodic acid-Schiff (PAS) technique (for neutral mucins). The potential differences between groups were desgined by applying the Whitney test and t test (p < 0.05). The crypts were smaller for the nickel-contaminated group, even though these organism exhibited broader and higher crypts. Nickel-contaminated animals exhibited a smaller amount of calyceform cells with AB and PAS positive reactions as well as a less mucus quantities when compared with nickel-uncontaminated animals. Such reductions on the amount of calyceform cells with AB and PAS positive reactions may be related wiht the shallower crypts, which possibly reduced the synthesis and secretion of mucins, compromissing the functional aspects (e.g., lubrification and intestinal mucosa protection) of the nickel-contaminated large intestines. Interestingly, the wider and higher crypts and higher epithelium collumn on the nickel-contaminated animals may represent a relevant trade-off for the intestinal mucosa protection.
镍慢性暴露对成年Wistar雄性大鼠大肠镜的影响
摄入大量被镍污染的水或食物会有很大的毒性。本研究旨在评价镍暴露对成年Wistar雄性大鼠升结肠的生理生理影响。我们使用了12只动物,将它们分为对照组(摄入未污染的水)和镍污染组(即25毫克镍/升的水)。氯化镍被提供在去污水中,实验有56天的暴露期。从动物的升结肠中取出一部分,并使用蓝甲苯胺(用于一般的生理测量描述)、阿利新蓝(AB,用于酸性粘蛋白)和周期性酸-希夫(PAS)技术(用于中性粘蛋白)进行生理标记。采用Whitney检验和t检验设计组间电位差异(p < 0.05)。镍污染组的隐窝较小,尽管这些生物显示出更宽和更高的隐窝。与未含镍的动物相比,镍污染动物的AB和PAS阳性的萼形细胞数量较少,粘液量也较少。AB和PAS阳性反应的盏状细胞数量的减少可能与隐窝较浅有关,这可能减少了粘蛋白的合成和分泌,损害了镍污染大肠的功能(如润滑和肠粘膜保护)。有趣的是,镍污染动物的更宽更高的隐窝和更高的上皮柱可能代表了肠黏膜保护的相关权衡。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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