Studies on Micro Propagation of Dracaena sanderiana Plants

Abstract

The experimental was intended to find out the well protocol for in vitro propagation of Dracaena sanderiana. In this respect, plant shoot tips were effectively surface sterilized with a mixture of sodium hypochlorite (NaOCl) as commercial Clorox and mercuric chloride (HgCl2) were used at 1.5 % NaOCl and 2.0 g/l HgCl2. Shoot tip explants were cultured on full MS-medium contains 1.5 mg/l IBA to establishment. For shoot multiplication, MS-medium contains 2 mg/l BA produced the highest number of shoots. For in vitro rooting, 2.0 mg/l IBA gave the highest number of roots and root length. Plantlets after rooting exhibited 100% survival in pots containing peatmoss and sand at a ratio of 3:1 under greenhouse conditions.