Super-resolution lensless microscopy using multi-wavelength multiplexing

Abstract

We report a multi-wavelength multiplexed setup and associated super-resolution reconstruction method in lensless microscopy, which can generate high-resolution reconstructions from undersampled raw measurements captured at multiple wavelengths. The reconstruction result of the Benchmark Quantitative Phase Microscopy Target (QPTTM) demonstrates the resolution enhancement quantitatively, which achieves a half-pitch lateral resolution of 691 nm across a large field of view (~29.85 mm2), surpassing 2.41 times of the theoretical NyquistShannon sampling resolution limit imposed by the pixel-size of the sensor (1.67 µm). Compared with other superresolution methods such as lateral or axial shift-based device and illumination source rotation design, wavelength multiplexed avoids the need for shifting/rotating mechanical components. This multi-wavelength multiplexed super-resolution method would benet the research and development of a more stable lensless microscopy system.