IDENTIFIKASI BAKTERI PATOGEN DAN PARASIT PENYEBAB PENYAKIT PADA IKAN TOMAN (Channa micropeltes)

Septyan Andriyanto, Hessy Novita, A. M. Lusiastuti, Taukhid Taukhid
{"title":"IDENTIFIKASI BAKTERI PATOGEN DAN PARASIT PENYEBAB PENYAKIT PADA IKAN TOMAN (Channa micropeltes)","authors":"Septyan Andriyanto, Hessy Novita, A. M. Lusiastuti, Taukhid Taukhid","doi":"10.15578/MA.15.1.2020.39-46","DOIUrl":null,"url":null,"abstract":"Penelitian ini bertujuan untuk mengetahui jenis patogen dan tingkat virulensi bakteri Aeromonas hydrophila pada ikan toman, Channa micropeltes. Penentuan jenis patogen dilakukan dengan identifikasi bakteri secara kimiawi dan menggunakan PCR. Hasil identifikasi bakteri pada ikan toman dengan uji biokimia mengarah pada Aeromonas hydrophila, Aeromonas salmonicida, Myroides sp., dan Edwardsiella sp. Begitupula hasil uji molekuler menggunakan PCR 16S rRNA seluruh isolat teridentifikasi sebagai bakteri. Spesies parasit yang teridentifikasi menginfeksi ikan toman dari golongan protozoa yaitu Trichodina sp., Vorticella sp., Henneguya sp., Oodinium sp., dan Ichthyophthirius multifiliis, parasit golongan trematoda yaitu Dactylogyrus sp. dan golongan acanthochepala yaitu spesies Acanthochepalus sp. Hasil uji virulensi melalui infeksi buatan menunjukkan bahwa bakteri Aeromonas hydrophila sangat virulen terhadap ikan toman, Channa micropeltes dengan tingkat kematian mencapai 100% dalam waktu 24 jam. The purpose of this study was to identify the pathogenicity and virulence of Aeromonas hydrophila in toman fish. General bacterial identification was carried out using biochemical analyzes and followed by PCR tests. Several pathogenic bacterial were found in the fish samples consisting of Aeromonas hydrophila, Aeromonas salmonicida, Myroides sp., and Edwardsiella sp. The PCR 16S rRNA test confirmed that all of the isolates were all bacteria. Parasites species identified in the samples of toman fish were Trichodina sp., Vorticella sp., Henneguya sp., Oodinium sp., Ichthyophthirius multifiliis, Dactylogyrus sp. and Acanthochepalus sp. The artificial infection treatment showed that toman fish was highly vulnerable to Aeromonas hydrophila, with 100% mortality within 24 hours.","PeriodicalId":334563,"journal":{"name":"Media Akuakultur","volume":"15 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Media Akuakultur","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15578/MA.15.1.2020.39-46","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Penelitian ini bertujuan untuk mengetahui jenis patogen dan tingkat virulensi bakteri Aeromonas hydrophila pada ikan toman, Channa micropeltes. Penentuan jenis patogen dilakukan dengan identifikasi bakteri secara kimiawi dan menggunakan PCR. Hasil identifikasi bakteri pada ikan toman dengan uji biokimia mengarah pada Aeromonas hydrophila, Aeromonas salmonicida, Myroides sp., dan Edwardsiella sp. Begitupula hasil uji molekuler menggunakan PCR 16S rRNA seluruh isolat teridentifikasi sebagai bakteri. Spesies parasit yang teridentifikasi menginfeksi ikan toman dari golongan protozoa yaitu Trichodina sp., Vorticella sp., Henneguya sp., Oodinium sp., dan Ichthyophthirius multifiliis, parasit golongan trematoda yaitu Dactylogyrus sp. dan golongan acanthochepala yaitu spesies Acanthochepalus sp. Hasil uji virulensi melalui infeksi buatan menunjukkan bahwa bakteri Aeromonas hydrophila sangat virulen terhadap ikan toman, Channa micropeltes dengan tingkat kematian mencapai 100% dalam waktu 24 jam. The purpose of this study was to identify the pathogenicity and virulence of Aeromonas hydrophila in toman fish. General bacterial identification was carried out using biochemical analyzes and followed by PCR tests. Several pathogenic bacterial were found in the fish samples consisting of Aeromonas hydrophila, Aeromonas salmonicida, Myroides sp., and Edwardsiella sp. The PCR 16S rRNA test confirmed that all of the isolates were all bacteria. Parasites species identified in the samples of toman fish were Trichodina sp., Vorticella sp., Henneguya sp., Oodinium sp., Ichthyophthirius multifiliis, Dactylogyrus sp. and Acanthochepalus sp. The artificial infection treatment showed that toman fish was highly vulnerable to Aeromonas hydrophila, with 100% mortality within 24 hours.
识别病因鱼的病原体和寄生虫
这项研究的目的是确定托曼、尚纳微颗粒中空气传播细菌的病原体的类型和水平。通过化学鉴定和使用PCR来确定类型的病原体。根据托曼鱼的生物化学测试,将其与水样、沙门氏菌、芽孢杆菌和芽孢菌结合进行鉴定,就像使用PCR 16S rRNA的分子检测检测发现的细菌一样。阶级的物种被确认感染的寄生虫鱼托曼原生动物,即Trichodina sp, Vorticella sp。Henneguya sp, Oodinium sp, Ichthyophthirius multifiliis,寄生虫trematoda即Dactylogyrus sp。阶级和阶级acanthochepala即Acanthochepalus sp物种。virulensi通过人工感染试验结果表明,细菌Aeromonas hydrophila对鱼托曼,Channa virulen micropeltes死亡率在24小时内达到100%。这项研究的目的是确定托曼鱼的病理和空气中的水样。常用生物化学分析和PCR测试进行跟踪。在鱼的样本中发现了几种病毒性杆菌,包括氢化物、鲑鱼皮菌、芽孢杆菌和Edwardsiella。在托曼鱼的样本中发现的物种是三周后的。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信