Production, Purification and Characterization of Terpenes Isolated From Two New Strains of Yeasts in Iraq and Study of Their Antiparasitic Activity

Abstract

The current study was included isolation and identification of yeasts from  five sediments soil samples  from Al-Faw  and Shatt al-Arab  in Basrah provinces, Iraq. The soil samples were collected during fallow period 1-1-2021 to 1-6-2022. In the primary isolation dilution method used for cultured soil samples, two crud colonies filtration was used. Two types of terpenes (T1, T2) were purified from culture filtrates  of both species Cystobasidium benthicum and C .minutum separately. The thin layer chromatography and column chromatography were used in the first step of purification. Cytotoxicity test  revealed no toxic effect for both terpenes  at 0.2 gm / ml concentration. The two terpenes were identified using ultra violet and infra-red spectroscopy as well. Therefore the results  showed the terpenes nature of both toxins and determination of important active groups in the structure of the two isolated terpenes. The current study aimed to search for more treatments that limit the vitality of the larval stages of the parasite, so it was done antiparasite activity for both terpenes were performed against Echinococcus granulosus the results showed the T1 more activity against parasite compared with T2.