A reliable technique for chronic carotid arterial catheterization in the rat

Journal of pharmacological methods Pub Date : 1991-07-01 DOI:10.1016/0160-5402(91)90033-2

Ban C.H. Tsui, Susan J. Mosher, Pollen K.F. Yeung

{"title":"A reliable technique for chronic carotid arterial catheterization in the rat","authors":"Ban C.H. Tsui, Susan J. Mosher, Pollen K.F. Yeung","doi":"10.1016/0160-5402(91)90033-2","DOIUrl":null,"url":null,"abstract":"<div><p>A reliable and simple method for cannulating the carotid artery of rats is described. The rat was anesthetized with halothane. The right carotid artery located between the omohyoideus and sternohyoideus muscles was exposed through a 2-cm ventral neck incision. The catheter was made of Silastic tubing and a monofilament line, which served as an obturator and internal support. The line was then filled with a viscous mixture of heparin, polyvinylpyrrolidinone and normal saline to prevent the formation of blood clots. The catheter was advanced through the carotid artery towards the heart by a predetermined distance (15–20 mm) depending on the size of the rat. The catheter was well tolerated by the rats and the success rate was 95%. Its patency lasted for at least 7 days postsurgery without any special maintenance care. With the described method one would be able to perform repetitive blood sampling and arterial blood pressure measurements in unanesthetized and unrestrained rats for prolonged period after Catheterization.</p></div>","PeriodicalId":16819,"journal":{"name":"Journal of pharmacological methods","volume":"25 4","pages":"Pages 343-352"},"PeriodicalIF":0.0000,"publicationDate":"1991-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0160-5402(91)90033-2","citationCount":"24","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of pharmacological methods","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0160540291900332","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 24

Abstract

A reliable and simple method for cannulating the carotid artery of rats is described. The rat was anesthetized with halothane. The right carotid artery located between the omohyoideus and sternohyoideus muscles was exposed through a 2-cm ventral neck incision. The catheter was made of Silastic tubing and a monofilament line, which served as an obturator and internal support. The line was then filled with a viscous mixture of heparin, polyvinylpyrrolidinone and normal saline to prevent the formation of blood clots. The catheter was advanced through the carotid artery towards the heart by a predetermined distance (15–20 mm) depending on the size of the rat. The catheter was well tolerated by the rats and the success rate was 95%. Its patency lasted for at least 7 days postsurgery without any special maintenance care. With the described method one would be able to perform repetitive blood sampling and arterial blood pressure measurements in unanesthetized and unrestrained rats for prolonged period after Catheterization.

查看原文本刊更多论文

一种可靠的大鼠慢性颈动脉插管技术

介绍了一种可靠、简便的大鼠颈动脉插管方法。用氟烷麻醉大鼠。右颈动脉位于肩胛舌肌和胸骨舌肌之间，通过2厘米的颈部腹侧切口暴露。导管由硅橡胶管和单丝线制成，作为封闭器和内部支撑。然后用肝素、聚乙烯吡咯烷酮和生理盐水的粘性混合物填充，以防止血栓的形成。根据大鼠的大小，导管通过颈动脉向心脏推进一段预定的距离(15 - 20mm)。大鼠对导管耐受良好，成功率为95%。术后通畅至少7天，无特殊维持护理。使用所描述的方法，可以在插管后长时间内对未麻醉和不受约束的大鼠进行重复的血液采样和动脉血压测量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of pharmacological methods

自引率

0.00%

发文量