Maintenance of cns tissues in vitro for subsequent pharmacologie evaluation: A simple and inexpensive superfusion chamber

Abstract

A new simple, inexpensive holding chamber is described for maintaining brain slices in a viable condition for long periods of time. The advantages of its superfusion-type operation and application of this chamber to in vitro electrophysiologic studies of the pharmacologie action of serotonin in the midbrain dorsal raphe nucleus are discussed.