On-Line Liquid Chromatography Detection by Photon Correlation Spectroscopy

Abstract

Photon Correlation Spectroscopy has been applied to the routine monitoring of proteins eluting off liquid chromatographic separation systems. The technique has been configured as an on-line, in situ detector capable of remote analyses using fibre optic technology. Analyses of flowing protein samples has been demonstrated and its use in process biochemistry as a quality assurance monitor is discussed.