Lipopeptide produced by the bacteria Bacillus mojavensis with activity against the phytopathogenic fungus Colletotrichum gloeosporioides Penz & Sacc var. Minor Simmonds

Abstract

The objective of this work was to determine the antifungal activity against Colletotrichum gloeosporioides Penz & Sacc var. minor Simmonds of the lipopeptide(s) isolated and purified from the culture of the marine bacterium Bacillus mojavensis (MC3B-22). The results will show that the extraction method with ammonium sulfate was the one that extracted the largest amount of biosurfactants with a yield of 3.1243 g/L compared to the acid precipitation method (0.3173 g/L). The crude extract in the presence of 103 conidia/mL of the fungus Colletotrichum gloeosporioides did not present inhibition, but the semi-purified extract obtained with ammonium sulfate reached a minimum inhibitory concentration of 12.5 µg/mL. In conclusion, the optimal method for extracting the biosurfactant was 40% ammonium sulfate, with methanol being a suitable solvent to semi-purify and obtain a minimum inhibitory concentration of 25 µg/mL against C. gloeosporioides.