Antimicrobial and Anticancer Activity of a Novel Peptide (Musterolysin) Extracted from Slurry of Mustard Oil Refinery Industry

Abstract

: Bacterial infections and cancer has been most prevalent diseases throughout the World. The discovery and development of new antibacterial and anticancer agents is critical. If such a valuable medicinal product is created from waste resources from the oil refinery business, academicians and industrialists will pay close attention. The sequential extraction process followed by RP-HPLC fractions was used to purify and identify a new peptide from mustard oil refinery sludge. The sequence of the peptide was determined as NH 2 -KYQFFVP-COOH using MALDI TOF/TOF Mass spectrometry. The antibacterial activity of the peptide was determined against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis , and Bacillus subtilis . The proapoptotic activity of the peptide was also determined against Hep2 (Human epidermoid cancer cell line). These findings suggest that readily available bio-waste might be a novel source of bioactive antibacterial and proapoptotic lipopeptides.