Fundamental study to apply the pulsed power technology on the biomass production

M. Yamanaka, A. Fujita, K. Teranishi, N. Shimomura
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Abstract

The depletion of fossil fuel is a serious global problem. The development of new biomass fuel, which is not made from foods as corn and sugar cane, is an urgent necessity. For the easiness of the cultivation and the richness for containing fuel, hydrophytic microalga is expected as a crude material for biomass fuel. The manufacturing process is sketchily classified into cultivation, extraction of oil component and refinement of fuel. The manufacturing process without cultivation is, however, inefficient at present. This study applies pulsed power technology to the production process of biomass fuel made from microalga, which is the cultivation and extraction process. Nanosecond pulsed electric fields are applied to the microalga to anticipate increment of cultivation speed and modification of component. In this experiment, the alga density of large number of treated samples was higher than that of negative control samples after seven days of the treatment. The technology to open up pores on cell membrane is called electroporation, and it is important cell control technology in the biotechnology. In another experiment, pulsed power was applied to the alga to improve the efficiency of component extracting process with electroporation. The pulsed powers with different pulse length were used to consider the effect.
脉冲功率技术在生物质生产中的应用基础研究
化石燃料的枯竭是一个严重的全球性问题。迫切需要开发新的生物质燃料,这种燃料不是由玉米和甘蔗等食物制成的。水生微藻因其易于培养和含燃料丰富,有望作为生物质燃料的原料。生产过程大致分为种植、提取油成分和精炼燃料。然而,目前没有种植的生产过程效率很低。本研究将脉冲功率技术应用于微藻生物质燃料的生产过程,即培养和提取过程。应用纳秒级脉冲电场对微藻进行培养速度的提高和成分的修饰。在本实验中,经过7天的处理后,大量处理样品的藻类密度高于阴性对照样品。在细胞膜上开孔的技术称为电穿孔,是生物技术中重要的细胞控制技术。在另一项实验中,对海藻施加脉冲功率,提高电穿孔提取过程中成分的效率。采用不同脉冲长度下的脉冲功率来考虑影响。
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