Environmental Design for Muscle Cell Culture with Magnetic Field

Abstract

Control methodology on cell culture has been studied for regenerative medicine and micro-actuator in an interdisciplinary research field. Environment has been designed around cultured muscle cells with a magnetic field, and an effect of the magnetic field at an extremely low frequency on proliferation and orientation of cultured skeletal muscle cells has been studied in vitro. Skeletal muscle cells were isolated from a hind-limb quadriceps, and cultured on a dish coated with collagen. The culture dishes were placed parallel to the uniform alternating magnetic field, which was generated with a solenoid coil. The result shows that the cultured skeletal muscle cells tend to align to the direction of the magnetic field at the extremely low frequency.