MORINGA OLEIFERA LEAVES AQUEOUS EXTRACT INDUCE APOPTOSIS IN HT29 CELL LINE

Abstract

Moringa oleifera (MO) tree has been recognized for the nutritional and medicinal values, attributing antiinflammatory, antioxidants, and antidiabetic activities of the alcoholic and/or water extracts to its various parts. Recent research has focused on therapeutic anti-cancerous effects of the water extract of MO leaves (MOE), commonly consumed as hot beverage. In-vitro cytotoxicity on esophageal, lung, and breast cancer cells were reported by MOE. The aim in this study is to investigate the anti-cancerous potential of MOE on the colon carcinoma HT29 cells. MO water extract (MOE) was prepared at concentrations (0.1%-0.5%). MOE anti-proliferative effect on HT29-cells was assessed by measuring cellular viability using trypan blue assay. MOE cytotoxicity was further examined by monitoring the change in levels of ROS (NBT assay), ATP (Luciferase assay), and LDH release (cytotoxicity assay) and on depolarization of mitochondrial membrane potential (Mito PTTMJC-1). Protective effect of N-Acetyl cysteine and catalase were assayed. Cell cycle analysis and Annexin-V/PI staining were also performed to detect cell death. Our result showed that MOE induced a dose and time-dependent decrease in viability; increase in ROS and LDH release; dissipation of mitochondrial membrane potential with concomitant decrease in ATP level. In addition, we obtained an increase in preG0 and late apoptotic events. Pretreatment with the antioxidant N-acetyl cysteine suppressed completely LDH release and restored partially and significantly cell viability while decreasing ROS level. Catalase was not protective. MOE also induced cell death with related colon cancer cell lines: Caco2, SW837, SW948, and HCT116. The induced apoptosis in HT29 cells and related CCC identified MOE with anti-cancerous therapeutic potential.