Gelatin Methacrylate Loaded with Adipose-Derived Mesenchymal Stem Cell Exosomes Promotes High-Quality Healing of Injured Tendon by Activating Both SMAD1/5/9 and SMAD2/3

EngRN: Pharmaceutical Engineering (Topic) Pub Date : 2021-01-07 DOI:10.2139/ssrn.3762214

Hengchen Liu, Mingzhao Zhang, Manyu Shi, Tingting Zhang, Wenjun Lu, Shulong Yang, Qingbo Cui, Zhaozhu Li

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Abstract

Adipose-derived mesenchymal stem cell exomes (ADSC-Exos) have become a new therapeutic target in biomedical field owing to their important role in regenerative medicine. However, the role of ADSC-Exos in tendon repair has not yet been evaluated. In this study, we explored a new treatment method for tendon injury, combining exosome therapy with local targeting of biohydrogel. We found ADSC-Exos could be absorbed by tendon stem cells (TSCs) and promoted the proliferation, migration, and tenogenic differentiation of the TSCs, which may have depended on the activation of the Smad2/3 and Smad1/5/9 pathways. Furthermore, gelatin methacrylate (GelMA) loaded with ADSC-Exos could promote initial healing of tendons and normalize tendon structure. Overall, we demonstrated that ADSC-Exos contributed to tendon regeneration and that ADSC-Exos–loaded GelMA was able to promote high-quality healing after tendon injury. This study provides proof of concept of a new idea for treating tendon injuries.

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装载脂肪来源的间充质干细胞外泌体的甲基丙烯酸明胶通过激活SMAD1/5/9和SMAD2/3促进损伤肌腱的高质量愈合

脂肪源性间充质干细胞外显子组(ADSC-Exos)因其在再生医学中的重要作用而成为生物医学领域新的治疗靶点。然而，ADSC-Exos在肌腱修复中的作用尚未得到评估。在本研究中，我们探索了一种新的肌腱损伤治疗方法，将外泌体治疗与生物水凝胶局部靶向相结合。我们发现ADSC-Exos可以被肌腱干细胞(TSCs)吸收，并促进TSCs的增殖、迁移和成肌腱分化，这可能依赖于Smad2/3和Smad1/5/9通路的激活。此外，装载ADSC-Exos的甲基丙烯酸明胶(GelMA)可以促进肌腱的初始愈合并使肌腱结构正常化。总的来说，我们证明了ADSC-Exos有助于肌腱再生，并且加载ADSC-Exos的GelMA能够促进肌腱损伤后的高质量愈合。本研究为治疗肌腱损伤提供了一种新思路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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EngRN: Pharmaceutical Engineering (Topic)

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