~(60)Co gamma-irradiation enhances expression of GAP-43 mRNA in rat brain

Abstract

Objective\ To study the relationship between the expression of GAP\|43 mRNA and nerve regeneration in rat brain after 60 Co γ\|irradiation. Methods\ Wistar rats were subjected to whole\|body irradiation with 8 Gy 60 Co γ\|rays.The expression of GAP\|43 was detected by in situ hybridization histochemistry using Dig\|cRNA probe. Results\ It was found that the expression of GAP\|43 mRNA increased in the cerebral cortex,caudate,putamen,globus pallidus,thalamus and hypothalamus one week after 8 Gy 60 Co γ\|irradiation.The peak of GAP\|43 mRNA expression was observed in the fourth week and then began to decrease but still remained at a higher than normal level.However,it decreased to a low level after 7 weeks. Conclusion\ Enhanced expression of GAP\|43 mRNA after 60 Co γ\|irradiation in rat brain is associated with nerve regeneration and reconstruction of synapse.