Design of Mass-Sensitive Sensor Array for Biomedical Application: Sensing Lipoproteins

Abstract

Herein we report the design of a novel sensor array based on molecularly imprinted polymer (MIP) for lowdensity lipoprotein (LDL) and high-density lipoprotein (HDL) determination. MIP of each lipoprotein class comprising comonomer of methacrylic acid (MAA) and vinyl pyrrolidone (NVP) in the ratio of 3:2 and 2:3, were screened onto threeelectrode quartz crystal microbalance (QCM) as selective elements toward LDL and HDL, respectively. Sensor array device reveals concentration-dependent sensor responses toward varying amount of each standard LDL and HDL solution with a linear correlation coefficient of R$^{2} =$ 0.89 and 0.99, respectively. HDL-MIP presents cross-reactivity to LDL at 9-19% compared to the LDL-MIP signal. LDL-MIP also has cross-reactivity to HDL at 0-3% of LDL-MIP responses. All sensor signals are fully reversible and the sensor array can be reused.