KERAGAMAN GENETIK Taxus SumatranaDARI POPULASI ALAM DI JAMBI MENGGUNAKAN PENANDA RAPD

Istiana Prihatini, Dodi Frianto, Eka Novriyanti, I. Nurtjahjaningsih, Aypbc Widyatmoko
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Abstract

The natural population of Taxus sumatrana in several areas in southern Asia, including Indonesia, has decreased in number. In Sumatra, populations of less than 20 trees/ha were found in the Gunung Kerinci and Gunung Tujuh areas, in Kerinci District, Jambi. Conservation efforts to protect this species from extinction are being carried out, for example, exploring seeds from natural populations for ex-situ plantations. The genetic diversity information of Taxus is required to develop conservation strategies for this species. This research aimed to observethe genetic diversity of Taxus from Gunung Tujuh and Gunung Kerinci populations in Jambi. The level of genetic diversity of 44 individuals from those populations was analyzed using ten markers of RAPD. The application of thosemarkers produced 104 loci used in data analysis using the GenAlex version 6.5 software. The result showed the heterozygosity value described the moderate level of genetic diversity of the two observed populations of 0,33±0.14 (Gunung Tujuh) and 0,35±0.14 (Gunung Kerinci). The genetic distance between the two populations (0,097) and genetic similarity (0,908) indicate that the two populations have a moderate variation in genetic structure. This study shows that the two populations have variations in geneticstructure at medium level although geographically close. Genetic conservation efforts for Taxus species in the future should involve more individuals from both populations and separate them as two different populations
从JAMBI的自然人口中发现了ta克萨斯苏门答腊的基因变异,使用了RAPD标记
在包括印度尼西亚在内的南亚几个地区,苏门答腊红豆杉的自然种群数量已经减少。在苏门答腊,占碑市Kerinci区的Gunung Kerinci和Gunung Tujuh地区发现的树木数量少于20棵/公顷。保护这一物种免于灭绝的努力正在进行中,例如,探索自然种群的种子用于移地种植。红豆杉的遗传多样性信息是制定保护策略所必需的。本研究旨在观察占碑地区Gunung Tujuh和Gunung Kerinci红豆杉种群的遗传多样性。利用10个RAPD标记分析了这些群体中44个个体的遗传多样性水平。这些标记的应用产生了104个位点,使用GenAlex 6.5版软件进行数据分析。结果表明,两个观察群体的杂合度值分别为0.33±0.14(古农·图朱)和0.35±0.14(古农·克林西),具有中等水平的遗传多样性。两个居群的遗传距离为0.097,遗传相似度为0.908,表明两个居群在遗传结构上存在中等差异。本研究表明,两个种群在地理位置上相近,但遗传结构上存在中等水平的差异。今后对红豆杉物种的遗传保护工作应包括更多的来自两个种群的个体,并将它们作为两个不同的种群进行分离
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