Reconstitution of biological clock into phospholipid-coated microdroplet

Abstract

In this paper, we try to establish newly technique that the components of the biological clock are reconstituted into the liposome. In other words, we try to produce a nano size clock, capsulated into the liposome, made by protein molecules. The biological clock is a basic cellular system found in almost all organisms. This clock generates self-sustained oscillations under constant conditions with a ^24-hour (circadian) period. Only three proteins in cyanobacteria reconstituted biological clock. We reconstitute these proteins and adenosine triphosphate (ATP) into phospholipid-coated microdroplet and confirmed the clock function. This system will reveal more detailed about biological clock system and bring application possibility, such as drug delivery.