Virulence Factors and Antibiotic Susceptibility Pattern of Acinetobacter Species in a Tertiary Care Hospital in Bangladesh

Ibrahim Medical College Journal Pub Date : 2013-04-23 DOI:10.3329/IMCJ.V6I1.14722

Azizun Nahar, S. Anwar, A. Saleh, R. Miah

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引用次数: 2

Abstract

Acinetobacter species are aerobic Gram variable coccobacilli that are now emerging as an important nosocomial pathogen. Infections caused by them are difficult to control due to multidrug resistance. The purpose of this study was to detect virulence factors namely gelatinase production, biofilm formation and antibiotic susceptibility of Acinetobacter species. Two hundred fifty six clinical samples collected from Bangabandhu Sheikh Mujib medical University (BSMMU) and from burn unit of Dhaka Medical College Hospital were included in the study. Gelatinase production was seen on Luria Bertani agar media containing gelatin (30 gm/l) and biofilm formation was detected in microtiter plate assay. Out of 256 clinical samples, 52 (20.3%) were Acinetobacter species . Out of 52 Acinetobacter isolates, none were gelatinase producer but 39 (75%) were found biofilm producers. Acinetobacter isolates were 100% resistant to ceftazidime, cefotaxime cefuroxime and ceftriaxone. High level of resistance was also recorded for amoxicillin (98.1%), aztreonam (98.1%), gentamicin (90.4%), ciprofloxacin (73.1%), amikacin (57.6%), netilmicin (53.8%) and imipenem (44.2%). Susceptibility to colistin was maximum (96.2%). The present study demonstrated a high propensity of biofilm formation by the clinical isolates of Acinetobacter species and most of the Acinetobacter were multidrug resistant. DOI: http://dx.doi.org/10.3329/imcj.v6i1.14722 Ibrahim Med. Coll. J. 2012; 6(1): 27-30

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孟加拉国一家三级医院不动杆菌种类的毒力因素和抗生素敏感性模式

不动杆菌是一种需氧革兰氏变球芽杆菌，目前已成为一种重要的医院病原体。由于耐多药，它们引起的感染难以控制。本研究的目的是检测不动杆菌的毒力因素，即明胶酶的产生、生物膜的形成和抗生素的敏感性。从孟加拉班班杜谢赫穆吉布医科大学(BSMMU)和达卡医学院附属医院烧伤科采集的256份临床样本被纳入研究。在含有明胶(30 gm/l)的Luria Bertani琼脂培养基上观察到明胶酶的产生，并用微滴板法检测生物膜的形成。256份临床样本中，52份(20.3%)为不动杆菌。在52株不动杆菌分离株中，没有一株产生明胶酶，但发现有39株(75%)产生生物膜。分离的不动杆菌对头孢他啶、头孢噻肟、头孢呋辛和头孢曲松耐药100%。阿莫西林(98.1%)、氨曲南(98.1%)、庆大霉素(90.4%)、环丙沙星(73.1%)、阿米卡星(57.6%)、那替米星(53.8%)、亚胺培南(44.2%)耐药程度较高。对粘菌素的敏感性最高(96.2%)。本研究表明临床分离的不动杆菌种类具有高度的生物膜形成倾向，并且大多数不动杆菌具有多重耐药性。DOI: http://dx.doi.org/10.3329/imcj.v6i1.14722 Ibrahim Med. col。j . 2012;6 (1):

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Ibrahim Medical College Journal

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