KATI ORTAM FERMANTASYON KOŞULLARINDA YENİ İZOLE EDİLMİŞ GANODERMA LUCIDUM İLE LAKKAZ ÜRETİMİ VE BOYA RENK GİDERİMİ İÇİN KULLANILMASI

Abstract

Laccase production capacity of newly isolated fungus Ganoderma lucidum was studied during solid state fermentation on solid substrates. Because wheat bran was detected as the most effective solid substrate, effect of various culture parameters and some inducers on laccase production in wheat bran media was investigated. Wheat bran with moisture content of 75%, pH 5.0 and 30 °C was the most effective medium and 10 mM copper addition supported the highest amount of laccase. In glass tray fermenter 2973±220 U/L laccase activity was obtained. Crude laccase gave the highest activity at 70 °C. It was stable at 60°C for 6 hours and retained 37% activity at 70 °C for 2 hours. It decolorized 61% of Remazol Brilliant Blue R within 20 min. Results showed that this strain could produce high amount of laccase and suitable inducer such as copper could induce its laccase production. The crude laccase obtained could be used for textile dye decolorization applications.