Meristem culture of Prunus avium cvs. “Siyah-e-Mashhad” and “Takdane”

Azarian Journal of Agriculture Pub Date : 2019-02-01 DOI:10.29252/AZARINJ.003

M. Zamanipour, E. G. Moghaddam, A. Tehranifar, B. Abedi

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Abstract

Accepted: 2 Feb. 2019 The present trial aimed to evaluate the effect of three media including WPM, MS and modified QL and three different hormone combinations (0, 0.5 and 1 mgL BAP) with 0.1 mgL GA3 and 0.1 mgL IBA on regeneration of two sweet cherry cultivars cvs "Siyah-e-Mashhad" and "Takdane". All experiments were arranged in completely randomized designed. Each treatment contained three replicates. The meristem explants were introduced in culture media supplemented with different hormonal combinations and maintained in a growth room at a 16h photoperiod (36 μmol.m.s), 25 ± 1oC. After six weeks, Survival, necrosis and contamination percent with the number of leaf were studied. The results showed that highest survival rate was in modified QL medium complemented with 1 mgL BAP and the least one was in MS medium without BAP treatments for both cultivars. Necrosis rate was high in MS (100-83.3%) in control for "Takdane" and "Siyah-e-Mashhad" cultivars, respectively that don’t have any differences with 0.5 mgL BAP. The most Leaf number (12) observed in modified QL medium supplemented with 1 mgL BAP in "Takdane" cultivar and the least one (2) was in MS medium without BAP application (control) in "Siyah-e-Mashhad" cultivar.

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鸟李的分生组织培养。" Siyah-e-Mashhad "和" Takdane "

本试验旨在评价WPM、MS和改性QL 3种培养基以及3种不同激素组合(0、0.5和1 mg / l BAP)与0.1 mg / l GA3和0.1 mg / l IBA对甜樱桃品种“siya -e- mashhad”和“Takdane”再生的影响。所有实验均采用完全随机设计。每个处理包含3个重复。将分生组织外植体引入添加不同激素组合的培养基中，在25±1℃的光照周期(36 μmol.m.s)下培养16h。6周后，观察叶片的存活率、坏死率和污染率随叶片数量的变化。结果表明，两个品种在添加1 mg BAP的改良QL培养基中存活率最高，在不添加BAP的MS培养基中存活率最低。对照品种“Takdane”和“Siyah-e-Mashhad”的MS坏死率较高(100-83.3%)，与0.5 mgL BAP差异不大。“Takdane”品种在添加1 mg BAP的改良QL培养基中观察到的叶片数最多(12个)，“Siyah-e-Mashhad”品种在不添加BAP的MS培养基(对照)中观察到的叶片数最少(2个)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Azarian Journal of Agriculture

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