Mechanism underlying the inhibitory effect of Elaeagnus Conferta Roxb on TNF –α induced NF- kB nuclear translocation in non- small lung cancer A549 cell line using Flow cytometry

G. Lalitha, N. Th
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引用次数: 1

Abstract

Our study examined for the inhibitory effect of ethanolic extract of Eleagnus Conferta Roxb leaves on TNF –α induced NFkB nuclear translocation in lung cancer A549 cell line using flow cytometry. Apoptosis also studied to know about the antiproliferative and anticancer effects. However, our results revealed in apoptosis, Elaeagnus conferta Roxb leaves showed (33.54%) increased in proportion of cells. In the study of pre-treatment of A549 cells with Elaeagnus conferta Roxb leaves followed by TNFα caused the increased proportion of cells (20.78%) at apoptosis induced cell death, which was statistically significant (p< 0.001). The untreated A549 cells had minimal NF-kB expression (0.25± 0.01%). However, the approach of A549 cells with Elaeagnus conferta Roxb had induced NF-kB production many fold (11.50± 1.05%). Therefore, we conclude our study was proved the impact of Elaeagnus conferta Roxb leaves inhibit the cellular growth of NSCLC-A549 cell line and induces apoptosis. Hence, from our findings, we proved this plant has anticancer activity, further feasibly taken for drug formulation .
用流式细胞术研究白菖蒲对TNF - α诱导的非小肺癌A549细胞NF- kB核易位抑制作用的机制
本研究采用流式细胞术检测了白菖蒲叶乙醇提取物对TNF -α诱导的肺癌A549细胞NFkB核易位的抑制作用。细胞凋亡也被研究以了解其抗增殖和抗癌作用。然而,我们的研究结果显示,在细胞凋亡方面,菖蒲叶的细胞比例增加(33.54%)。在研究中,荷叶预处理A549细胞后,TNFα使凋亡诱导的细胞死亡比例增加(20.78%),差异有统计学意义(p< 0.001)。未经处理的A549细胞NF-kB表达最低(0.25±0.01%)。然而,与Elaeagnus conferta Roxb接触的A549细胞可诱导NF-kB产生数倍(11.50±1.05%)。因此,我们认为本研究证实了菖蒲叶对NSCLC-A549细胞系细胞生长的抑制作用和诱导细胞凋亡的作用。因此,从我们的研究结果,我们证明了这种植物具有抗癌活性,进一步可行的药物配方。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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