Molecular Analysis of Pomphorhynchus kashmirensis based on 18S rDNA and ITS-rDNA

International Journal of Zoology and Animal Biology Pub Date : 1900-01-01 DOI:10.23880/izab-16000372

Amin Om

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Abstract

Pomphorhynchus kashmirensis Kaw, 1941 was redescribed to correct inadequacies in the original description. We provide a molecular characterization to qualify its now completed morphological description and to compare with related taxa. Sequences of the 18S rDNA and ITS-rDNA datasets of specimens of P. kashmirensis were generated for assessment of gene diversity and the phylogenetic analyses. Comparative sequence analysis indicated interspecific variation between P. kashmirensis with different species of Pomphorhynchus was 0.2-1.4% and 2.4-36.4% based on 18S rDNA and ITS-rDNA datasets, respectively. The ITS-rDNA region is more variable than 18S rDNA within members of the family Pomphorhynchidae and it appears appropriate for assessment of their biodiversity. Our phylogenetic analyses showed that taxonomic position of the species P. kashmirensis is closely related with P. tereticollis and P. laevis. Also, the systematic status of Tenuiproboscis and Longicollum is uncertain within the family Pomphorhynchidae. Therefore, further molecular investigations will be needed for better understanding of the phylogenetic relationships in this family.

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基于18S rDNA和ITS-rDNA的喀什米尔水蚤分子分析

重新描述了1941年《克什米尔法》，以纠正原始描述中的不足之处。我们提供了一个分子表征，以确定其现已完成的形态描述，并与相关分类群进行比较。对喀什米尔猿人标本的18S rDNA和ITS-rDNA数据集进行序列分析，用于基因多样性评估和系统发育分析。比较序列分析结果表明，18S rDNA和ITS-rDNA数据显示，克什米尔人与不同种Pomphorhynchus的种间差异分别为0.2 ~ 1.4%和2.4 ~ 36.4%。在Pomphorhynchidae科成员中，ITS-rDNA区域比18S rDNA区域更具变异性，似乎适合于评估其生物多样性。系统发育分析表明，喀什米兰的分类学地位与普特拉科斯和拉维斯密切相关。此外，tenuiprobosis和Longicollum在Pomphorhynchidae科中的系统地位也不确定。因此，需要进一步的分子研究来更好地了解该家族的系统发育关系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal of Zoology and Animal Biology

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