{"title":"What is the diagnostic value of beta-D-glucuronidase (BDG) activity of bacteria using Fluorocult ECD agar for their cultivation?","authors":"B Ralovich, G A Ibrahim, A Fábián, M Herpay","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A total of 1510 strains from 15 genera of Gram-negative and Gram-positive bacteria were studied. More than 94% of 327 Escherichia coli strains showed beta-D-glucuronidase (BDG) activity. Seventeen serotypes from 170 E. coli O serogroup representatives were negative. Relationship between the existence of BDG positive and negative E. coli strains in the same serogroup or serotype has not been observed. The rate of BDG positivity was 42% among Salmonella arizonae strains and 42.2% among Shigella strains. Only one Citrobacter strain out of the 971 strains belonging to Citrobacter, Edwardsiella, Enterobacter, Hafnia, Klebsiella, Proteus, Serratia, Yersinia, Pseudomonas, Aeromonas, Vibrio and Listeria was BDG positive. A screening method based on only BDG activity is not sufficient for the primary diagnosis of E. coli.</p>","PeriodicalId":76970,"journal":{"name":"Acta microbiologica Hungarica","volume":"38 2","pages":"147-54"},"PeriodicalIF":0.0000,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta microbiologica Hungarica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A total of 1510 strains from 15 genera of Gram-negative and Gram-positive bacteria were studied. More than 94% of 327 Escherichia coli strains showed beta-D-glucuronidase (BDG) activity. Seventeen serotypes from 170 E. coli O serogroup representatives were negative. Relationship between the existence of BDG positive and negative E. coli strains in the same serogroup or serotype has not been observed. The rate of BDG positivity was 42% among Salmonella arizonae strains and 42.2% among Shigella strains. Only one Citrobacter strain out of the 971 strains belonging to Citrobacter, Edwardsiella, Enterobacter, Hafnia, Klebsiella, Proteus, Serratia, Yersinia, Pseudomonas, Aeromonas, Vibrio and Listeria was BDG positive. A screening method based on only BDG activity is not sufficient for the primary diagnosis of E. coli.
使用Fluorocult ECD琼脂进行细菌培养，检测- d -葡萄糖醛酸酶(BDG)活性的诊断价值是什么?
共对革兰氏阴性菌和革兰氏阳性菌15属1510株进行了研究。在327株大肠杆菌中，有超过94%的菌株具有β - d -葡萄糖醛酸酶(BDG)活性。170个O型大肠杆菌血清组代表中有17个血清型呈阴性。在同一血清组或血清型中存在BDG阳性和阴性大肠杆菌菌株之间的关系尚未观察到。亚利桑那沙门氏菌BDG阳性率为42%，志贺氏菌阳性率为42.2%。971株Citrobacter、Edwardsiella、Enterobacter、Hafnia、Klebsiella、Proteus、Serratia、Yersinia、Pseudomonas、Aeromonas、Vibrio和Listeria菌株中仅有1株呈BDG阳性。仅基于BDG活性的筛选方法不足以对大肠杆菌进行初步诊断。
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