Biofilm formation by Methicillin resistant Staphylococcus aureus and its relation to antibiotic resistance in Thi-qar province/Iraq

Abstract

Methicillin resistant Staphylococcus aureus is one of the most dangerous pathogens in the community and the hospital environment for its high resistance to antibiotics and the production of a number of virulence agents such as toxins and biofilm. The study aims to determine the ability of local isolates to produce the biological membrane and its relationship to resistance to antibiotics. A cross sectional study include (37) isolates of methicillin-resistant S. aureus from the burn department at Al-Hussein Teaching Hospital in Thi Qar province/Iraq for the period April-October 2015, The capacity of bacteria to produced biofilm was done by micro plate technique and the antibiotic susceptibility test for vancomycin, amikacin, ciprofloxacin, norfloxacin, cefotaxime and amoxicillin-Claviolinate using the diffusion technique of antibiotic disks. The study showed that 64.9% of the MRSA isolates were able to form biofilm, while the isolates were fully resistant to the used beta-lactam antibiotics. But, the 94.6% of the isolates were sensitive to the vancomycin. There was no significant statistical relationship between the antibiotic resistance and the ability of bacteria to produce the biofilm except for the ciprofloxacin. The study showed that the local MRSA isolates have a high ability to produce the biological membrane and antibiotic resistance with the exception of the vancomycin with a relationship between the resistance to ciprofloxacin and the production of the biological membrane by bacteria. Therefore, the study recommends the use of vancomycin in medical sites to treat the infections caused by MRSA to prevent the spread and development of these resistant strains.