Comparative GC-MS Analysis of Phytochemicals from Clitorea ternatea L. (White Flower Variety) Leaves Treated with Planetary Sounds (Planet Earth and Planet Jupiter) and Estimation of Bioactive Compounds

Aditya Kumar Dash, D. Rath, S. Padhi
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Abstract

The butterfly pea (Clitoria ternatea L.) is a plant with various potential health benefits due to the presence of many bioactive compounds. The leaves contain triterpenoids, flavonoids, glycosides, and alkaloids. In the present experimental work, the leave extracts treated with planetary sound (Sound of Earth and Sound of Jupiter) classified as Medium-low frequency sound waves (200 Hz to 1 KHz), in methanol were checked for bioactive components through GC-MS and the third set was kept as control. Different bioactive compounds identified, were further estimated by using Lupeol (Merck, India) as an external standard. In compounds common among plants treated with Earth’s sound and control, 3-O-Methyl-d-glucose from plants treated with Earth’s sound was highest at 1.20 PPM. Compounds common among plants treated with Jupiter’s sound and control, 3-Hexenal from plants treated with Jupiter’s sound was found to be highest with 1.87 PPM, Similarly, compounds common among plant’s treated with Earth’s sound and Jupiter’s sound, Gamma sitosterol from leaves of plants treated with Earth’s sound was found to have highest value i.e, 1.27 PPM. Compounds that were common for all the three groups, plants treated with Earth’s sound was found to have increased Squalene with 1.23 PPM. A general trend of phytochemicals from plants treated with planet Earth’s sound had an elevated value. This suggests that the treatment of planet Earth’s sound has tremendous impact on the production of secondary metabolites in the referred species.
白花品种阴蒂(Clitorea ternatea L.)叶片经行星声(地球和木星)处理后植物化学成分的GC-MS对比分析及生物活性成分的估算
蝶豆(Clitoria ternatea L.)是一种具有多种潜在健康益处的植物,因为它含有许多生物活性化合物。叶子含有三萜、类黄酮、糖苷和生物碱。本实验以分类为中低频声波(200 Hz ~ 1 KHz)的行星声(地球声和木星声)处理的叶提取物,在甲醇中通过GC-MS检测其生物活性成分,并以第三组为对照。通过使用Lupeol (Merck, India)作为外部标准进一步估计鉴定出的不同生物活性化合物。在地球声处理和对照植物中常见的化合物中,来自地球声处理植物的3- o -甲基-d-葡萄糖含量最高,为1.20 PPM。用木星的声音处理和对照的植物中常见的化合物,用木星的声音处理的植物中常见的3-己烯醛被发现最高,为1.87 PPM,同样,用地球的声音和木星的声音处理的植物中常见的化合物,用地球的声音处理的植物的叶子中的伽马谷甾醇被发现具有最高的值,即1.27 PPM。三组植物中常见的化合物,经过地球声音处理的植物中角鲨烯的含量增加了1.23 PPM。从经过地球声音处理的植物中产生的植物化学物质的总体趋势具有升高的值。这表明地球声音的处理对参考物种次生代谢物的产生有巨大的影响。
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