Plant Comparative Transcriptomics Reveals Functional Mechanisms and Gene Regulatory Networks Involved in Anther Development and Male Sterility

Xiangyuan Wan, Ziwen Li
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引用次数: 7

Abstract

Gene transcription and transcriptional regulation are crucial biological processes in all cellular life. Through the next-generation sequencing (NGS) technology, transcriptome data from different tissues and developmental stages can be easily obtained, which provides us a powerful tool to reveal the transcriptional landscape of investigated tissue(s) at special developmental stage(s). Anther development is an important process not only for sexual plant reproduction but also for genic male sterility (GMS) used in agriculture production. Plant comparative transcriptomics has been widely used to uncover molecular mechanism of GMS. Here, we focused on researches of anther developmental process and plant GMS genes by using comparative transcriptomics method. In detail, the contents include the following: (1) we described the commonly used flowchart in comparative transcriptomics; (2) we summarized the comparative strategies used to analyze transcriptome data; (3) we presented a case study on a maize GMS gene, ZmMs33 ; (4) we described the methods and results previ-ously reported on gene co-expression and gene regulatory networks; (5) we presented the workflow of a case study on gene regulatory network reconstruction. The further development of comparative transcriptomics will provide us more powerful theoretical and application tools to investigate molecular mechanism underlying anther development and plant male sterility.
植物比较转录组学揭示花药发育和雄性不育的功能机制和基因调控网络
基因转录和转录调控是所有细胞生命中至关重要的生物学过程。新一代测序(NGS)技术可以很容易地获得不同组织和发育阶段的转录组数据,为揭示特定发育阶段所研究组织的转录格局提供了有力的工具。花药发育不仅是植物有性生殖的重要过程,也是农业生产中遗传雄性不育的重要组成部分。植物比较转录组学已被广泛用于揭示GMS的分子机制。本文主要利用比较转录组学方法对植物花药发育过程和GMS基因进行研究。具体内容包括:(1)描述了比较转录组学中常用的流程图;(2)总结了用于分析转录组数据的比较策略;(3)以玉米GMS基因ZmMs33为例;(4)对基因共表达和基因调控网络的研究方法和结果进行了综述;(5)介绍了基因调控网络重构案例研究的工作流程。比较转录组学的进一步发展将为研究植物花药发育和雄性不育的分子机制提供更有力的理论和应用工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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