The Effect of Optimum Photoperiod from Blue LED Light on Growth of Chlorella Vulgaris in Photobioreactor Tank

Narong Kamolrat, Settakorn Kamuang, Thiamthep Khamket, P. Sangmek, S. Sitthaphanit
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Abstract

Abstract Study for investigated the optimum photoperiod from blue LED light for the growth of Chlorella vulgaris in culture tank. Photoperiods from blue LED Light (Light/Dark) were set up at 24:0, 16:8 and 12:12 h. The initial number of C. vulgaris cells was 1.6 x 105 ± 0.12 cells mL-1. Culture medium was using commercially available fertilizers. After 17 days of cultivation, the results showed that the 24:0 h photoperiod had better growth of algae than 16:8 and 12:12 h, with maximum number of cells of 1.64 x 107 ± 0.23 cells mL-1 specific growth rate (SGR) was 0.17 ± 0.05, -0.06 ± 0.11 and -0.19 ± 0.1% day-1 with statistically significant differences (P < 0.05) in day 5 of cultivation. The stationary phase continued up to day 3 of cultivation, between the treatment. The optimum temperature for growth ranged from 35.5–38.5°C. The results showed that blue LED lighting at 24:0 h is the optimum photoperiod for cultivation of C. vulgaris. Keywords: Photobioreactor tank, Photoperiod, Blue LED light, Chlorella vulgaris, Growth rate
蓝光LED最佳光周期对普通小球藻在光生物反应器池中生长的影响
摘要研究了蓝色LED光对小球藻生长的最佳光周期。蓝光LED光周期(Light/Dark)分别于24:0、16:8和12:12 h设置,初始细胞数为1.6 × 105±0.12个细胞mL-1。培养基使用市售肥料。培养17 d后,结果表明:24:0 h光周期的藻类生长情况优于16:8和12:12 h,最大细胞数为1.64 × 107±0.23个细胞mL-1的特定生长率(SGR)分别为0.17±0.05、-0.06±0.11和-0.19±0.1% day-1,培养第5天差异有统计学意义(P < 0.05)。在两次处理之间,固定期一直持续到培养的第3天。生长的最佳温度为35.5 ~ 38.5℃。结果表明,蓝光LED照明时间为24:0 h,是黄姜栽培的最佳光周期。关键词:光生物反应器槽,光周期,蓝色LED灯,小球藻,生长速率
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