Synthesis and Characterization of Silver Nanoparticles using Padina pavonica Extract and Evaluation of Their Antibacterial Activity

Abstract

Nanoparticles are the basic essential elements in the wall of nanotechnology and it exhibits fabulous advanced characteristic features based on their properties such as size, morphology and other size dependent properties. In this study, marine algae Padina pavonica (Linn.) was used for the biosynthesis of silver nanoparticles. The biosynthesized silver nanoparticles were characterized with UV-vis spectroscopy, FTIR and SEM. The SEM analysis showed the particle size between 20-70 nm as well as the polydispersed spherical structure of the nanoparticles. The extract of marine alga P. pavonica (Linn.) as well as silver nanoparticles were tested against gram positive Staphylococcus aureus, Bacillus subtilis and gram negative bacteria as Escherichia coli and Pseudomonas aeruginos . The P. pavonica based silver nanoparticles significantly inhibited the growth of bacteria. The AgNPs possess potential antibacterial activity than algae extract. This novel highly stable spherical polydispersed Ag nanoparticles were synthesized using a simple environmental friendly green method and can be used for antibacterial agent.