Effect of plant growth regulators on micro-propagation of selected Sri Lankan traditional and improved rice cultivars (Oryza sativa L.)

D. M. P. Dissanayaka, N. Dahanayake
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引用次数: 2

Abstract

In this study five selected Sri Lankan traditional rice varieties (Kaluheenati, Sulaai, Suwadhal, Dostara Heenati) (Oryza sativa L.) and five improved rice varieties (At362, Bg94/1, Bg358, Bg357, Bg379) were examined for callus induction, plant regeneration and rooting using in vitro techniques. Seeds were cultured in five different hormone concentrations; 1.0, 1.5, 2.0, 2.5, 3.0 mg/l 2, 4- Dichlorophenoxy acitic acid (2, 4-D) and 0.1 mg/l 6 – Benzyl adenine purine (BAP) on Murashige and Skoogs (MS) basal medium for callus induction. Proliferated calli were transferred to five different hormone concentrations; 1.0, 1.5, 2.0, 2.5, 3.0 mg/l BAP with 0.1 mg/l Indole acetic acid (IAA) to regenerate shoots. Regenerated shoots were transferred to three different hormone concentrations; 1.5, 2.0, 3.0 mg/l Indole butric acid (IBA) for rooting. Complete Randomized Design (CRD) was used with ten replicates and data was analyzed by SAS 9.1.3 version. The interaction between plant varieties and different hormone concentrations are highly significant for callus diameter, number of shoots per explants and root length. All tested rice cultivars (100%) were able to produce callus however only 40% of rice varieties were regenerate shoots. The regeneration ability of rice varieties varied from 0% to 100%. All shoots were able to produce 100% roots in tested IBA concentrations. Among all selected rice varieties 2.0 mg/l 2, 4-D+0.1 mg/l BAP is most successful for callus induction while 2.0 mg/l BAP+0.1 mg/l IAA and 2.0 mg/l IBA most effective for shoot regeneration and root induction respectively. DOI:  http://dx.doi.org/10.4038/suslj.v13i1.7671 Sabaragamuwa University Journal 2014; V. 13 No. 1 pp 33-41
植物生长调节剂对斯里兰卡传统和改良水稻品种微繁的影响
选用斯里兰卡5个传统水稻品种(Kaluheenati、Sulaai、Suwadhal、Dostara Heenati) (Oryza sativa L.)和5个改良水稻品种(At362、Bg94/1、Bg358、Bg357、Bg379),采用离体培养技术对愈伤组织诱导、植株再生和生根进行了研究。种子在五种不同的激素浓度中培养;1.0、1.5、2.0、2.5、3.0 mg/l 2,4 -二氯苯氧基酸(2,4 - d)和0.1 mg/l 6 -苄基腺嘌呤(BAP)在Murashige和Skoogs (MS)基础培养基上诱导愈伤组织。将增殖的愈伤组织转移到五种不同的激素浓度中;1.0、1.5、2.0、2.5、3.0 mg/l BAP加0.1 mg/l吲哚乙酸(IAA)再生嫩枝。将再生芽转移到三种不同浓度的激素中;1.5、2.0、3.0 mg/l吲哚丁酸(IBA)用于生根。采用完全随机设计(CRD),共10个重复,数据分析采用SAS 9.1.3版本。不同激素浓度对愈伤组织直径、外植体芽数和根长有显著的互作效应。所有水稻品种(100%)均能产生愈伤组织,但只有40%的水稻品种再生芽。水稻品种的再生能力在0% ~ 100%之间。在测试的IBA浓度下,所有的芽都能产生100%的根。2.0 mg/l 2,4 - d +0.1 mg/l BAP诱导愈伤组织效果最好,2.0 mg/l BAP+0.1 mg/l IAA和2.0 mg/l IBA诱导愈伤组织再生效果最好。DOI: http://dx.doi.org/10.4038/suslj.v13i1.7671 Sabaragamuwa University Journal 2014;第13节,第33-41页
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