Title: Effect of Curcumin and Ginger on Adverse Effects of Levofloxacin in Male Rats

H. Ibrahim, Shimaa M. Abdallah, Esraa Maghawry Abd-Allah El-Gazzar
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引用次数: 1

Abstract

Fluoroquinolones are more likely than any other antibacterial drug classes to cause serious side effects. The purpose of this study is to assess the protective effects of vitamin E, curcumin, and ginger in male rats against the side effects of levofloxacin (LFX).Ninety male Wistar rats were placed into six groups, each of 15 animals. Groups 1 and 2 received distilled water or olive oil and kept as normal controls, while groups 3-6 received LFX [10 mg/kg body weight (BW)] alone or in combination with vitamin E (100 mg/kg BW.), curcumin (200 mg/kg BW.) or ginger (200 mg/kg BW.), respectively. All medications were administered orally via gavage once a day for 5 successive days. Samples of blood were collected at zero day , 7th and 14th day posttreatments for biochemical analysis, while liver and kidney tissues were dissected and subjected to antioxidants estimation. Levofloxacin administration caused hepatic and renal damage evidenced by significant increase in serum liver enzymes, urea (76.43±3.70, 72.67±0.47, 75.00±6.42), creatinine (2.48±0.01, 2.48±0.01, 2.50±0.01), total cholesterol (166.67±6.81, 174.00±4.04, 176.00±2.88), triglycerides (269.67±5.84, 289.33±4.91, 249.67±1.86), and low density lipoproteincholesterol (LDLC) (243.00±7.09, 213.67±5.03, 226.33±5.45) levels. Meanwhile, significant decline in serum levels of high density lipoproteincholesterol (HDLC) (20.00±1.11, 23.33±3.38, 20.10±1.53), total protein (5.77±0.03, 5.97±0.09, 5.87±0.09), albumin (3.45±0.03, 3.60±0.06, 3.57±0.09) and A/G ratio (1.49±0.02, 1.51±0.05, 1.55±0.09) were determined in relation to normal control at zero, 7th and 14th day post-treatments, respectively in a time dependent manner. The superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities in the liver (1.50±0.13, 1.67±0.18, 1.60±0.15; 2.02±0.38, 2.81±0.25, 3.30±0.37) and kidney (24.97±1.04, 21.33±1.33, 26.33±1.71; 3.15±0.14, 2.13±0.36, 2.44±0.28) tissues were significantly decreased in all levofloxacinexposed rats compared to control values in the liver (3.00±0.11, 3.33±3.13, 3.33±0.16 for SOD; 19.76±0.03, 19.18±0.05, 18.42±0.13 for GPx) and kidney (71.33 ± 2.60, 67.67±0.88, 66.00±1.73 for SOD; 8.32±0.08, 8.30±0.04, 7.81±0.06 for GPx). However, the hepatic and renal malondialdehyde (MDA) concentrations (74.51±0.69, 80.97±0.41, 95.88±0.52; 66.58±0.14, 65.09±0.12, 65.23±0.14) were significantly increased compared to the control (11.82±0.01, 11.82±0.001, 11.11±0.03; 30.88±0.03, 33.20±0.03, 32.83±0.04).Co-administration of LFX with vitamin E, curcumin or ginger attenuated the elevated liver enzymes, renal damage biomarkers, lipogram and hepato-renal MDA, with elevation of tissues antioxidants. From this study, biochemical results indicated that levofloxacin induced hepato-renal alterations in rats over time, and that curcumin or ginger medications may be more effective when used prophylactically rather than curatively.
题目:姜黄素和生姜对左氧氟沙星对雄性大鼠不良反应的影响
氟喹诺酮类药物比其他抗菌药更容易引起严重的副作用。本研究的目的是评估维生素E、姜黄素和生姜对雄性大鼠左氧氟沙星(LFX)副作用的保护作用。90只雄性Wistar大鼠被分成6组,每组15只。1组和2组分别给予蒸馏水或橄榄油作为正常对照组,3-6组分别给予LFX [10 mg/kg体重]单独或与维生素E (100 mg/kg体重)、姜黄素(200 mg/kg体重)或生姜(200 mg/kg体重)联合治疗。所有药物均口服灌胃给药,每天1次,连续5天。分别于治疗后第0天、第7天和第14天采集血液进行生化分析,同时解剖肝脏和肾脏组织进行抗氧化剂测定。左氧氟沙星引起肝肾损害,血清肝酶、尿素(76.43±3.70、72.67±0.47、75.00±6.42)、肌酐(2.48±0.01、2.48±0.01、2.50±0.01)、总胆固醇(166.67±6.81、174.00±4.04、176.00±2.88)、甘油三酯(269.67±5.84、289.33±4.91、249.67±1.86)、低密度脂蛋白胆固醇(LDLC)(243.00±7.09、213.67±5.03、226.33±5.45)水平显著升高。同时,血清高密度脂蛋白胆固醇(HDLC)(20.00±1.11,23.33±3.38,20.10±1.53)、总蛋白(5.77±0.03,5.97±0.09,5.87±0.09)、白蛋白(3.45±0.03,3.60±0.06,3.57±0.09)和A/G比(1.49±0.02,1.51±0.05,1.55±0.09)水平在治疗后第0天、第7天和第14天与正常对照组相比均显著下降,且呈时间依赖性。肝脏超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GPx)活性分别为1.50±0.13、1.67±0.18、1.60±0.15;2.02±0.38,2.81±0.25,3.30±0.37)和肾(24.97±1.04,21.33±1.33,26.33±1.71;与对照组相比,左氧氟沙星暴露大鼠肝脏SOD(3.00±0.11,3.33±3.13,3.33±0.16)显著降低(3.15±0.14,2.13±0.36,2.44±0.28);GPx为19.76±0.03,19.18±0.05,18.42±0.13),肾SOD为71.33±2.60,67.67±0.88,66.00±1.73;GPx为8.32±0.08,8.30±0.04,7.81±0.06)。肝脏和肾脏丙二醛(MDA)浓度分别为74.51±0.69、80.97±0.41、95.88±0.52;66.58±0.14、65.09±0.12、65.23±0.14),与对照组(11.82±0.01、11.82±0.001、11.11±0.03;30.88±0.03,33.20±0.03,32.83±0.04)。LFX与维生素E、姜黄素或生姜联合使用可降低升高的肝酶、肾损伤生物标志物、脂质图和肝肾MDA,同时提高组织抗氧化剂。从这项研究中,生化结果表明,随着时间的推移,左氧氟沙星会引起大鼠的肝肾改变,姜黄素或生姜药物在预防而不是治疗时可能更有效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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