Identification of Molecular Marker Based on MYB Transcription Factor for the Selection of Indonesian Fine Cacao (Theobroma cacao L.)

L. Devy, Indah Anita-Sari, Tengku Imam Saputra, A. Susilo, A. Wachjar, Sobir
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引用次数: 3

Abstract

Indonesia is the third largest cacao (Theobroma cacao L.) producer in the world and also well-known for its fine cacao varieties (Java fine-flavor cacao). Indonesian fine cacao breeding program will be accelerated by early detection of its specific trait through the use of molecular marker. One of the traits thatcould differentiate fine and bulk cacao, in this case Criollo and Forastero, respectively, is the pod color. Previous research reported that MYB transcription factor gene regulated cacao pod color and was able to differentiate Criollo from Forastero. The gene involved in the control of plant-specific processes including primary and secondary metabolism, cell fate and identity, developmental processes and responses to biotic and abiotic stresses. This research aimed to identify the diversity of Indonesian fine and bulk cacao based on MYB nucleotide sequence fragments. Identification of the MYB nucleotide sequence was conducted by DNA isolation from cacao leaves and specific primer design based on two cacao MYB transcription factor ene accessions. These primers were used to evaluate the diversity of three Indonesian fine cacao (DRC 16, PNT 16, and ICCRI 01) and two bulk cacao (PA 191 and ICCRI 03) clones. The cluster analysis showed that this specific primer is similar to other MYB gene accessions in Malvaceae family (Theobroma, Herrania, Gossypium). It is also able to differentiate bulk and fine cacao in accordance to their pedigree. The primer developed in this study could be used for further analysis of Indonesian fine cacao molecular marker.
基于MYB转录因子的印尼优质可可选育分子标记鉴定
印度尼西亚是世界上第三大可可生产国,也以其优良的可可品种(爪哇精味可可)而闻名。利用分子标记技术对印尼优质可可的性状进行早期检测,将加快印尼优质可可的育种进程。区分优质可可和散装可可的特征之一是豆荚的颜色,分别是克里奥罗和福拉斯特罗。先前有研究报道MYB转录因子基因调控可可豆荚颜色,并能区分Criollo和Forastero。该基因参与控制植物特异性过程,包括初级和次级代谢、细胞命运和身份、发育过程以及对生物和非生物胁迫的反应。本研究旨在基于MYB核苷酸序列片段鉴定印尼细可可和散装可可的多样性。从可可叶片中分离MYB核苷酸序列,并基于两个可可MYB转录因子基因的特异引物设计进行鉴定。利用这些引物对3个印尼优质可可(DRC 16、PNT 16和ICCRI 01)和2个散装可可(PA 191和ICCRI 03)的多样性进行了评价。聚类分析表明,该引物与Malvaceae家族(Theobroma, Herrania, Gossypium)中其他MYB基因加入物相似。它也能够区分散装和细可可根据他们的血统。该引物可用于印尼细可可分子标记的进一步分析。
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