GENOTYPE OF Cryptosporidium spp. ISOLATED FROM BOVINE OF AL-QADISIYAH PROVINCE /IRAQ

Abstract

The current research included examination of 100 fecal sample from bovine was collected from AL-Qadisiyah province, from September 2018 until February 2019.The Microscopically result showed that oval or spherical shaped with dark pink color or red oocyst on blue ground and 30(30%) positive sample out 100 case . It was recorded that the maximum rate 41.66% (5/12) was seen in November, but the lowest rate 18.75% (3/16) was seen in the month of February with no significant differences at level (p<0.05.) According to age the maximum rate of incidence 40%(14/35)was found in the age group lower than a month, but the lowest incidence was seen in the group( >1 years). There is no significant differences at p<0.05.between male and female. In the currently study the N-PCR in molecular investigation were used ,the positive sample was18 (60%) out of 30 fecal sample. Sequencing of a fragment of the(18s rRNA) gene (834 bp) that separated from many distinct area in AL- Qadisiyah government recorded (50%)6/12 sample related to NCBI – Blast Cryptosporidium parvum ,( 33.33%) 4/12 sample display deep related to NCBI –Blast Cryptosporidium bovis ( this first study reported C. bovis in Iraq ) , (16.66%) 2/12 sample showed closed related to NCBI –Blast Cryptosporidiumandersoni.