In vitro seed propagation of endangered Dendrobium - Dendrobium lituiflorum Lindl. and D. aduncum Lindl.

Kuntala Neog Barua, Bebija L. Singha, Bonanya Bora, Satyam Bordoloi
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Abstract

Genus Dendrobium of Orchidaceae prized for their floricultural excellence, that have fascinated the commercial growers all over the world and attention has paid to exploit this group. Destruction of specific habitats encourage the species vanished and need attention to conservation and restoration of rare and endangered taxa. In vitro seed propagation is the most popular multiplication technique and indispensable components of plant genetic resource management. Present observation deals with the effect of different media on In vitro seed proliferation and growth of plantlets of two endangered Dendrobium – D. lituiflorum Lindl. and D. aduncum Lindl. for large scale propagation. In D. lituiflorum, the highest germination percentage (95.48%) was recorded in MS medium supplemented with yeast extract, otherwise, in D. aduncum, 90.43 % germination was recorded in medium supplemented with potato extract. Quick proliferation of D. lituiflorum by the use of banana extract exhibited 12.5±1.77 number of shoot and 12.5±1.77 length. However, maximum number of shoots as 14.5±1.77 and length 4.84±0.58 cm. recorded in D. aduncum. After 12 weeks of culture, the medium containing 1.5 mgL-1 IBA supplemented with 0.2% banana extract showed maximum root formation (5.9±0.95) in D. lituiflorum. Otherwise, in D. aduncum, the highest number of roots7.9±1.33 per shoot was recorded the medium supplemented with 1.5mgL-1 IBA and 0.2% potato extract after 9 week of culture. In green house condition the seedlings of D. lituiflorum showed better performance in the medium containing brick chips: charcoal: coconut husk (1:1:2) and brick chips: charcoal: sphagnum moss (1:1:2) is better for D. aduncum. Seed derived propagation is very easy approach for multiplication of these species and could be successfully applied for mass multiplication intended for future conservation, and commercial aspects.
濒危石斛-石斛离体种子繁殖研究。D. aduncum Lindl。
兰科石斛属因其优良的花卉栽培而备受世界各地的商业种植者的青睐,并引起了人们的重视。特定生境的破坏助长了物种的消失,需要注意稀有和濒危分类群的保护和恢复。种子离体繁殖是目前应用最广泛的育种技术,是植物遗传资源管理的重要组成部分。本文研究了不同培养基对两种濒危石斛(D. lituflorum Lindl)离体种子增殖和植株生长的影响。D. aduncum Lindl。用于大规模传播。在MS培养基中添加酵母浸膏的柳花菊的发芽率最高,为95.48%,在添加马铃薯浸膏的培养基中,杜鹃的发芽率最高,为90.43%。用香蕉提取物快速增殖,其芽数为12.5±1.77,芽长为12.5±1.77。最大芽数为14.5±1.77,长为4.84±0.58 cm。记载于D. aduncum。培养12周后,在含有1.5 mg -1 IBA和0.2%香蕉提取物的培养基中,金盏花生根率最高(5.9±0.95)。在添加1.5mg -1 IBA和0.2%马铃薯提取物的培养基中,培养9周后,aduncum每枝生根数最高,为7.9±1.33根。在温室条件下,柳花丹幼苗在砖屑:木炭:椰子皮(1:1:2)和砖屑:木炭:泥沼苔藓(1:1:2)培养基中表现较好。种子繁殖是一种非常简单的繁殖方法,可以成功地应用于未来保护和商业方面的大量繁殖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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