Mutation Analysis of Second Primary Tumors in the Head and Neck Cancer by Next Generation Sequencing

Abstract

More than 90% of malignant tumors in the head and neck are squamous carcinomas. These patients are with an average survival rate of about 5 years. However, some of the head and neck cancer(HNC) patients had the poor survival rate because of development of second primary tumors. In this study, the sequencing was performed using the Illumina system and Sanger sequencing was used to validate all identified mutations. We analyzed primary and second primary tumors in HNC and identified 23 mutant verification only in second primary tumors; 32 mutant verification only in primary tumors; 38 mutant verification in both of them. This mutant verification only in second primary tumors might be the cause of the second primary oral cancer.