Structural delving and insilico analysis of proteins of Rhinacanthus Nasutus- an indigenous medicinal plant

Abstract

Medicinal plants are staging a comeback and ‘renaissance’ is happening all over the globe. The Medicinal plants products today symbolise safety in contrast to the synthetics that are regarded as unsafe to human and environment. So the medicinal plants have a promising role in to prevent and as well as to cure the diseases. Andrographis is an important genus of the family Acanthacae known for its ethanomedicinal claims and for a variety of medicinal properties. Rhinacanthus nasutus has been used in various folk medicinal preparations and its chemical composition and pharmacological activities have been elaborated recently. Functional characterization of a protein sequence is one of the most frequent problems in biology. This task is usually facilitated by accurate three-dimensional (3-D) structure of the protein. The number of protein sequences that can be modeled, as well as the accuracy of the prediction, is increasing steadily because of the growth and number of known protein sequences and structures as well as improvements in the modeling software. It is currently possible to model, with useful accuracy. Significant parts of approximately one half of all known protein sequences. This research report render a futuristic summary of bioinformatics study of Rhinacanthus nasutus  with emphasis on the current development and future directions, which shall provide tools and resources necessary to understand and uphold advances in this important field. The aim of the present study, 2 proteins of Rhinacanthus nasutus were analysed using bioinformatics tools. Structural prediction and functional characterization of proteins of Rhinacanthus nasutus were done using Expasy Protparm server, 3D structure was done using SWISS MODEL. Plants of different family showing identity 80% and above were selected and its sequences retrieved, aligned using Clustal Omega. phylogenetic tree was constructed for the aligned sequence.  Structure prediction showed that α – helix, random coil, β – turn and extended strand predominates. Phylogenetic analysis of rbcl of Rhinacanthus nasutus reveals that the Plants of Verbenaceae, Bignonaceae, and Fabaceae family are closely related. Hence the present article includes the detailed exploration of Computational tools of protein, and bioinformatic aspects of R.nasutus in an attempt to provide a direction for further research.