SEASONAL VARIATION OF FATTY ACID COMPOSITION OF PHOSPHOLIPID SUBCLASSES IN MUSCLE TISSUE IN MALE, ALBURNUS MUSSULENSIS

veysi kıymaz
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Abstract

Fatty acids are quite important both as an energy source and in terms of functional tasks for living systems. In addition, these compounds are the structures that have the greatest change depending ecological factors in terms of biochemical. These biochemical compounds are located in the cell structure. So, Analysis of these fatty acids, which have very important membrane integrity and permeability in life, is very important for living things. Therefore, fatty acid analysis at the phospholipid level is quite important. In this study, it was aimed to determine the seasonal variation of the fatty acids of phospholipid subclasses in muscle tissue. In our study, sexes of the samples caught from Batman Dam Lake were determined in the laboratory. After the total lipid extraction of the muscle tissue belonging to the male one was made, it was separated into phospholipid subclasses by thin layer chromatography. After the methylation process, samples were analyzed qualitatively as % fatty acid with Gas Chromatography. As a result of analysis, a total of 16 fatty acids were determined. As a results of analyses, out of saturated fatty acids, 16:0 and 18:0; out of monosaturated ones, 16:1n-7 and 18:1n-9; out of polyunsaturated ones, 20:4n-6, 20:5n-3 22:5n-322:5n-3 and 22:6n-6 were identified as the major fatty acids. When the fatty acid distribution is examined, 16:0 in PC; 18:0 in PI and PS; 18:1n-9 in PC and PS; in 20:4n-6 PI; 20:5n-3 in PC and PI; 22:6n-3 and n-3 in PE and n-6 were found to be higher in PI than others. It is detected that in April, total PUFA and n-3 in PC, PE and PI; in November and January, total SFA in PSs are higher than the other months. In our study, the PI subclasses n-3/n-6 rate of the fish has been at the lowest level according to PC, PE and PS.
雄性肌肉组织磷脂亚类脂肪酸组成的季节变化
脂肪酸无论是作为能量来源还是在生命系统的功能任务方面都是非常重要的。此外,在生物化学方面,这些化合物是受生态因素影响变化最大的结构。这些生化化合物位于细胞结构中。因此,对这些在生命中具有非常重要的膜完整性和通透性的脂肪酸进行分析对生物是非常重要的。因此,在磷脂水平上进行脂肪酸分析是非常重要的。本研究旨在确定肌肉组织中磷脂亚类脂肪酸的季节性变化。在我们的研究中,从蝙蝠侠水坝湖捕获的样本的性别是在实验室确定的。提取雄性肌肉组织的总脂质后,用薄层色谱法将其分成磷脂亚类。甲基化处理后,用气相色谱法定性分析样品为%脂肪酸。经分析,共鉴定出16种脂肪酸。根据分析结果,饱和脂肪酸中,16:0和18:0;单饱和的,16:1n-7和18:1n-9;在多不饱和脂肪酸中,20:4n-6、20:5n-3 22:5n-322:5n-3和22:6n-6是主要的脂肪酸。检测脂肪酸分布时,PC为16:0;PI和PS为18:0;PC和PS为18:1n-9;在20:4n-6 PI;PC和PI为20:5n-3;[22:6] PE和n-6中n-3和n-3的PI值较高。检测到4月份PC、PE和PI中PUFA和n-3总量;在11月和1月,ps的SFA总量高于其他月份。在我们的研究中,根据PC, PE和PS,鱼的PI亚类n-3/n-6率处于最低水平。
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