[A method of rapid indication of an antigen].

Abstract

A simple and sensitive method for rapid detection of an antigen is offered. 0.025 ml of antiserum is placed on the slide, 0.025 ml of clarified extract containing the antigen added, and then 0.025 ml of intact 20.10(6) suspension of Kowan 1 Staphylococcus strain is added. When antigen-antibody complex is forming, staphylococcal cell aggregation starts. A staphylococcal reagent, manufactured by the Pasteur Research Institute of Epidemiology and Microbiology in Leningrad, may be employed, this making the method more available. The indication sensitivity is 0.2-1.25 mln cells per ml, this value depending on the bacterial species.