PENGARUH PENAMBAHAN EKSTRAK BAWANG MERAH (Allium cepa) DALAM PENGENCER TRIS - KUNING TELUR TERHADAP KUALITAS SPERMATOZOA SAPI BALI PADA PENYIMPANAN IN VITRO (The effect of additional onion (Allium cepa) extract in tris - egg yolk extender on the sperm...

JURNAL NUKLEUS PETERNAKAN Pub Date : 2021-12-14 DOI:10.35508/nukleus.v8i2.4304

Fian K. Djita, W. M. Nalley, T. Hine, A. Marawali

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Abstract

The purpose of this study was to determine the effect of adding onion extract (OE) to Tris-egg yolk (T-EY) diluent on the quality of bali bulls spermatozoa. The semen of three bali bulls aged 3-4 years were collected twice a week using the artificial vaginal method. Semen with good quality, motility 79%, viability 84.63%, abnormality 2.93% diluted with T-EY diluent with the addition of OE: T0: 0%; T1 1%; T2: 2%; T3: 3%; T4: 4%; and T5: 5%. The diluted semen was preserved in a refrigerator at 3-5 °C and evaluated for the quality of the spermatozoa every 24 hours. The results showed that the addition of OE in T-EY diluent had a positive effect on the quality of bali bulls spermatozoa. The addition 3% of OE (T3) in T-EY diluent resulted in higher spermatozoa quality (P <0.05) than the other treatments, namely motility 42.01%, viability 48.74%, and abnormalities 4.13% on the sixth day of preservation. It was concluded that the addition of onion extract in Tris - egg yolk diluents could improve the quality of bali bulls spermatozoa, with the best level of onion extract being 3%.

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本试验旨在研究在三卵黄(T-EY)稀释液中添加洋葱提取物(OE)对巴利公牛精子质量的影响。采用人工阴道法采集3头3 ~ 4岁巴厘公牛的精液，每周2次。精液质量好，活力79%，活力84.63%，异常2.93%，经T-EY稀释，OE: T0: 0%;T1 1%;T2: 2%;T3: 3%;T4: 4%;T5: 5%。稀释后的精液保存在3-5°C的冰箱中，每24小时评估一次精子的质量。结果表明，在T-EY稀释剂中添加OE对巴利公牛精子质量有积极影响。在T-EY稀释液中添加3% OE (T3)的精子质量高于其他处理(P <0.05)，保存第6天精子活力42.01%，活力48.74%，异常率4.13%。综上所述，在蛋黄稀释液中添加洋葱提取物可提高巴厘公牛精子的质量，洋葱提取物的最佳添加水平为3%。

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JURNAL NUKLEUS PETERNAKAN

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