Conception of Impedance Spectroscopy Analyzer and validation using yeast cell

Abstract

The characterization of biological cells is of major importance in several research fields. Impedance Spectroscopy is the most used tool to characterize cells; it can be used to investigate electrical cells proprieties, to differentiate between different kinds of cells, and to quantify cells. The goal of this work is the conception of an Impedance Spectroscopy Analyzer for cell characterization. The yeast cells were used to validate the reliability and the accuracy of this analyzer. In our instrument, a small alternating current with a variable frequency is injected into the explored biological system by using two electrodes. Then, the same electrodes are used to acquire a potential difference which is used to characterize the biological tissue. The analyzer can be used to investigate the electrical properties of cell suspensions such as the blood and the cytoplasm. The designed device is automated by LabVIEW interface through a GPIB.