Current Trends in Toxin Analysis of Agricultural Products: Improving the Food Safety with the Help of Biotechnologies

T. Godjevargova
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Abstract

Food safety has become an extremely important problem for modern society. With increasing pollution of agricultural products with various toxic compounds, the need for technological control and consumer health protection has increased [1]. Therefore, new methods for sensitive and rapid on-site detection of toxic contaminants in food and agricultural products are required. The aim of this thematic issue is to carry out the detection of various toxic contaminants in food and agricultural products, and also the development of new sensitive methods for their detection. Pathogenic microorganisms such as Aspergillus flavus, Fusarium langsethia, and Staphylococcus aureus and their toxic metabolite compounds – aflatoxins, T-2 toxins, and enterotoxins, respectively, are present in agricultural products and are dangerous for human health [2]. Furthermore, pathogenic microorganisms cause mastitis in cows. During the onset of mastitis, neutrophils get activated, attracted and attempt to kill the invading pathogens [3]. Studying the role of neutrophils in the infection and its subsequent effect on some of the milk quality parameters is a very important task. Also, organophosphorus pesticides play an important role in agriculture, but they are very toxic compounds and they need constant monitoring [4]. The necessity of wide screening control of food and agriculture contamination is due to the widespread distribution and the significant and multiple toxic effects of these toxic compounds. The high toxicity of these toxins requires accurate and reliable detection methods for safety food, agricultural products and water. It is necessary to develop rapid and sensitive assays for the determination of low concentrations of these toxic compounds. Therefore, different new sensitive methods for the determination of the mentioned toxins are presented in this thematic issue. All the proposed assays are based on an immunoreaction antibody-antigen that is highly specific and sensitive [5]. A rapid and sensitive fluorescent immunoassay based on Magnetic Nanoparticles (MNPs) with a polyclonal and monoclonal antibody for deter-mination of enterotoxin A
农产品毒素分析的发展趋势:利用生物技术提高食品安全
食品安全已成为现代社会一个极其重要的问题。随着各种有毒化合物对农产品的污染日益严重,对技术控制和消费者健康保护的需求日益增加[1]。因此,需要新的方法对食品和农产品中的有毒污染物进行敏感和快速的现场检测。本期专题的目的是对食品和农产品中的各种有毒污染物进行检测,并开发新的敏感检测方法。农产品中存在黄曲霉、langsedium镰刀菌、金黄色葡萄球菌等致病微生物及其有毒代谢产物黄曲霉毒素、T-2毒素、肠毒素等,对人体健康具有危害[2]。此外,病原微生物引起奶牛乳腺炎。在乳腺炎发病期间,中性粒细胞被激活、吸引并试图杀死入侵的病原体[3]。研究中性粒细胞在感染过程中的作用及其对牛奶品质参数的影响是一项非常重要的任务。此外,有机磷农药在农业中发挥着重要作用,但它们是毒性很强的化合物,需要持续监测[4]。食品和农业污染的广泛筛选控制的必要性是由于这些有毒化合物的广泛分布和显著和多重毒性作用。这些毒素的高毒性需要准确可靠的检测方法来保证食品、农产品和水的安全。有必要开发快速、灵敏的检测方法来测定这些低浓度的有毒化合物。因此,在这个专题问题中提出了不同的新的敏感方法来测定上述毒素。所有提出的检测方法都是基于高度特异性和敏感性的免疫反应抗体抗原[5]。基于多克隆抗体和单克隆抗体的磁性纳米颗粒(MNPs)快速、灵敏的荧光免疫分析法测定肠毒素A
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