Development of 3-Photon Functional Imaging in Deep Layers of Macaque Cortex

Abstract

3-photon excitation laser-scanning microscopy enables fluorescence imaging deep in tissue beyond the range of 2-photon microscopy. Here, we report results of functional calcium imaging of neurons with 3-photon microscopy in non-human primates. We compare 2-photon and 3-photon excitation and characterize the performance of 3-photon microscopy at depths beyond the 2-photon limit.