Beta-adrenergic receptor stimulates L-type calcium current in adult smooth muscle cells.

Blood vessels Pub Date : 1991-01-01 DOI:10.1159/000158855
A Welling, J Felbel, K Peper, F Hofmann
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引用次数: 8

Abstract

The hormonal regulation of L-type calcium current was investigated in freshly isolated tracheal smooth muscle cells using the whole-cell configuration of the patch-clamp technique. Isoproterenol stimulated the L-type calcium current 2.6-fold through beta-adrenoceptors. Dialysis of these cells with cyclic AMP, cyclic AMP analogues or the catalytic subunit of cyclic AMP kinase had no effect on basal or isoproterenol-stimulated calcium current. The calcium current was stimulated and inhibited by dialysis of the cells with GTP gamma S and GDP beta S, respectively. Evidently, in some smooth muscle cells the beta-adrenoceptor couples directly to L-type calcium channels via a G protein.

肾上腺素能受体刺激成人平滑肌细胞的l型钙电流。
利用膜片钳技术的全细胞结构研究了新分离气管平滑肌细胞中l型钙电流的激素调节。异丙肾上腺素通过β -肾上腺素受体刺激l型钙电流2.6倍。用环AMP、环AMP类似物或环AMP激酶的催化亚基透析这些细胞对基础或异丙肾上腺素刺激的钙电流没有影响。GTP γ S和gdpβ S分别刺激和抑制钙电流。显然,在一些平滑肌细胞中-肾上腺素能受体通过G蛋白直接偶联到l型钙通道。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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