Polyacrylamide micro-pillar based DNA microarray and its application for genetic variation analysis.

E. S. Selamat, P.Y. Hong, W. Liu
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Abstract

To improve diffusive capabilities and target accessibility with a gel-based microarray, a novel `pads within a pad' approach was developed. Using a soft lithography technique, an array of `waffle'-like micro-pillars embedded on the surface of the glass slide substratum was fabricated. The micro-pillar based microarray showed more than a 3-fold increase in the effective surface area available for probe immobilization. As a result, a fivefold increase was observed in the hybridization rate and intensity of the `waffle' pad as compared to an unmodified gel pad. The functionality of the `waffle' pad was demonstrated by the accurate genotyping of two sets of SNPs (SNP_ 1236 and SNP_2677) based on the dissociation curve, dissociation temperature, Td and the difference of Td between the perfect match duplex with that of the mismatch (DeltaTd)
基于聚丙烯酰胺微柱的DNA芯片及其在遗传变异分析中的应用。
为了提高凝胶微阵列的扩散能力和目标可及性,开发了一种新的“垫中垫”方法。利用软光刻技术,在玻璃载玻片基底表面嵌入了一排“华夫饼”状的微柱。基于微柱的微阵列显示探针固定的有效表面积增加了3倍以上。结果,与未经修饰的凝胶垫相比,“华夫饼”垫的杂交率和强度增加了五倍。通过对两组snp (snp1236和SNP_2677)的解离曲线、解离温度、Td以及完美匹配双链与不匹配双链的Td差(DeltaTd)进行精确的基因分型,验证了“华夫饼”的功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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