A study of connective tissue metabolites in biological fluids of patients with various types of glaucoma

E. Stepanova, O. I. Lebedev
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Abstract

   PURPOSE. To quantify the content of connective tissue metabolites reflecting the exchange of its main components in biological fluids in low tension glaucoma (LTG) and primary open-angle glaucoma (POAG), and to conduct a comparative analysis of the obtained data.   METHODS. The studies were conducted in 33 patients with low tension glaucoma aged 51 to 80 years old (mean age 69 ± 10.4 years), 20 patients with POAG aged 53 to 80 years old (mean age 67 ± 10.1 years) and 15 clinically healthy volunteers aged 52 to 78 years old (mean age 68 ± 9.2 years). The protein components of the fibrous part of the connective tissue were analyzed for daily excretion of oxyproline in urine by the method involving para-Dimethylamin-obenzaldehyde modified by P.N. Sharaev. Serum glycosaminoglycans (GAG) were studied by analyzing carbohydrate components of connective tissue. The concentration of GAG in the blood serum was determined by the colorimetric method, using Dische's carbazole reaction.   RESULTS. The amount of GAG in the blood serum of LTG patients was 3.26 ± 1.57 mmol/L, while in the control group consisting of clinically healthy volunteers — 2.92 ± 1.25 mmol/L. The differences were not statistically significant (p > 0.1). The amount of daily excretion of oxyproline in the urine of LTG patients was 17.17 ± 8.64 mmol/day, and in the group of clinically healthy individuals — 13.96 ± 5.61 mmol/day. The differences were statistically significant (p < 0.05). The amount of GAG in the blood serum of POAG patients was 3.97 ± 1.17 mmol/L. The differences in relation to LTG were statistically significant (p < 0.05). The amount of daily excretion of oxyproline in the urine of POAG patients was 32.71 ± 19.79 mmol/day. The differences were statistically significant in relation to LTG (p < 0.05). The amount of GAG and oxyproline in biological fluids tends to increase with the advancement of LTG and POAG.   CONCLUSION. Destruction processes in the connective tissue do occur in LTG, but are less pronounced in comparison to POAG and more pronounced in comparison to healthy individuals. The indicator that most clearly reflects the activity of destructive processes in the connective tissue is the daily excretion of oxyproline in urine.
不同类型青光眼患者生物体液中结缔组织代谢产物的研究
目的。量化低压型青光眼(LTG)和原发性开角型青光眼(POAG)患者生物体液中反映其主要成分交换的结缔组织代谢物的含量,并对所得数据进行对比分析。方法。研究对象为33例51 ~ 80岁的低压性青光眼患者(平均年龄69±10.4岁)、20例53 ~ 80岁的POAG患者(平均年龄67±10.1岁)和15例临床健康志愿者(平均年龄68±9.2岁)。用P.N. Sharaev修饰的对二甲胺-苯甲醛法对结缔组织纤维部分的蛋白质成分进行了每日尿中羟脯氨酸排泄的分析。通过分析结缔组织碳水化合物组分,研究血清糖胺聚糖(GAG)含量。用比色法测定血清中GAG的浓度,并用Dische咔唑反应测定。结果。LTG患者血清中GAG含量为3.26±1.57 mmol/L,而临床健康志愿者组成的对照组为- 2.92±1.25 mmol/L。差异无统计学意义(p < 0.01)。LTG患者尿中羟脯氨酸日排泄量为17.17±8.64 mmol/d,临床健康组为- 13.96±5.61 mmol/d。差异有统计学意义(p < 0.05)。POAG患者血清中GAG含量为3.97±1.17 mmol/L。与LTG相关的差异有统计学意义(p < 0.05)。POAG患者尿中羟脯氨酸日排泄量为32.71±19.79 mmol/d。与LTG相关,差异有统计学意义(p < 0.05)。随着LTG和POAG的进展,生物体液中GAG和羟脯氨酸的含量有增加的趋势。结论。结缔组织的破坏过程确实发生在LTG中,但与POAG相比,结缔组织的破坏过程不那么明显,而与健康个体相比,结缔组织的破坏过程更为明显。最清楚地反映结缔组织中破坏性过程活动的指标是尿中氧脯氨酸的每日排泄。
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