[The IGSS (immunogold silver stain) method in the study of the immunophenotype of free cells. Electron microscopy study of human venous blood].

Abstract

In the present study a pre-embedding immunoelectron microscopical method ideally suited to detect suspended cell surface-associated antigens, is described. In this method 5nm colloidal gold particles have been enlarged by means of silver enhancement yielding a large marker that is easily detectable at the TEM level. The present method is particularly suited when investigation are performed with a low percentage of labeled cells as well as low antigen expression.